《植物生理学报》 2012, 48(5): 511-517

超低温保存植物种质资源的新途径——小滴玻璃化法

吴昀¹, 张琳¹, 林田², 夏宜平^1,*

1浙江大学农业与生物技术学院园林研究所, 杭州310058; 2上海市农业科学院农业生物基因中心, 上海201106

通信作者：夏宜平；E-mail: ypxia@zju.edu.cn；Tel: 0571-88982391

摘　要：

超低温保存是一种安全、有效的种质资源保存途径, 可长期保存种质资源。小滴玻璃化法是在滴冻法和玻璃化法上 基础上发展起来的用于植物种质资源保存的新技术。本文综述了该方法的技术概念、主要优点、基本程序、应用前景及 国内外研究现状。

关键词：小滴玻璃化法; 超低温保存; 种质资源

收稿：2012-01-17   修定：2012-04-13

资助：国家“863”计划子项目(2011AA100208)。

A Novel Approach for Cryopreservation of Plant Germplasm Resources—Droplet-Vitrification

WU Yun¹, ZHANG Lin¹, LIN Tian², XIA Yi-Ping^1,*

¹Institute of Landscape Architecture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; ²Shanghai Agriculture and Biology Gene Center, Shanghai Academy of Agricultural Science, Shanghai 201106, China

Corresponding author: XIA Yi-Ping; E-mail: ypxia@zju.edu.cn; Tel: 0571-88982391

Abstract:

Cryopreservation is a safe and efficient method for germplasm conservation, which could be long- term preservation. Droplet-vitrification technique is the newest method based on droplet-freezing method and vitrification method for plant germplasm resources cryopreservation. The present paper primally reviewed the concept, the major advantages, the basic procedure, the present research status at home and abroad, and application prospect.

Key words: droplet-vitrification; cryopreservation; plant